Device
TARS-LacI

Part:BBa_K108030:Design

Designed by: Shan Lin   Group: iGEM08_Tsinghua   (2008-10-23)

TARS-LacI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 31


Design Notes

TARS sequence was designed according to reference 1.

LVA sequence was designed according to reference 2.

PCR using primers with biobrick prefix and surfix, and then cloned into pSB1AC3.

Source

LacI operator, LacI and T7 terminator was cloned from pET-15b(Novagen).

References

1. An Artifical Aptazyme-Based Riboswitch and its Cascading System in E.coli. ChemBioChem. 2008, 9:206-209.

2. New Unstable Variants of Green Fluorescent Protein for Studies of Transient Gene Expression in Bacteria. APPLIED AND ENVIRONMENTAL MICROBIOLOGY. 1998, 64(6):2240–2246.