Composite

Part:BBa_K3037008

Designed by: Arnau Pérez Roig   Group: iGEM19_TU_Dresden   (2019-10-15)
Revision as of 23:07, 20 October 2019 by ArnauP (Talk | contribs) (→‎Sequence)

MBP+HRP

MBP+HRP
Function Expression
Use in Escherichia coli
RFC standard Freiburg RFC25 standard
Backbone pSB1C3
Submitted by Team: TU_Dresden 2019



Overview

The TU Dresden 2019 team design this fusion protein in order to increase the expression of HRP. In order to make it the RFC 25 standard was used (more information).

HRP was inserted into the pSB1C3 vector for transformation and expressed in Escherichia coli.

The HRP was made from BBa_K1800002 but adapted to the RFC 25 standard

Characterization

Outline

1) Expression in pOCC97, growing curve (BBa_3037000)

2) Expression in pOCC97, SDS-PAGE (BBa_3037000)

Experiments in Detail

Expression in pOCC97, growing curve (BBa_3037000)

  • Growing curve (Check that the growing curve of the bacteria is not affected by the expression of the protein)

Expression in pOCC97, SDS-PAGE (BBa_3037000)

  • SDS-PAGE (Comparison of expression of different proteins in the vector before and after optimization)

Sequence

NOTE: For some reason the specified scar when aploaded the sequence was the one of the RFC25 standard but the registry shows the one of the RFC23


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1357
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 381
    Illegal BglII site found at 1599
    Illegal XhoI site found at 1683
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 79


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Categories
Parameters
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