Composite

Part:BBa_K3165057

Designed by: Mohit Das   Group: iGEM19_IISc-Bangalore   (2019-10-20)
Revision as of 17:17, 20 October 2019 by Registry (Talk | contribs)

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T7 - nMag + T7 - pMag + mOrange (for Bacillus subtilis)

This composite system consists of the entire optogenetic T7 RNA Polymerase system with mOrange under the pCpcG2 promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 828
    Illegal BsaI site found at 1723
    Illegal BsaI.rc site found at 2257
    Illegal BsaI.rc site found at 2296


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Parameters
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