RNA

Part:BBa_K3081104

Designed by: Le Zhang   Group: iGEM19_Peking   (2019-10-16)
Revision as of 14:18, 20 October 2019 by Lelezhang (Talk | contribs)


scaffold of sgRNA in CRISPR/Cas system

This part is composed of a 42-nt-long dCas9 handle hairpin for Cas9 protein binding and a 40-nt-long transcription terminator hairpin derived from S. pyogenes.When designing a sgRNA for CRISPR/Cas9 system, you just need to put the target sequence before the sgRNA scaffold.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None