RNA

Part:BBa_K3081104

Designed by: Le Zhang   Group: iGEM19_Peking   (2019-10-16)


scaffold of sgRNA in CRISPR/Cas system

This part is composed of a 42-nt-long dCas9 handle hairpin for Cas9 protein binding and a 40-nt-long transcription terminator hairpin derived from S. pyogenes. When designing a sgRNA for CRISPR/Cas9 system, you just need to put the target sequence before the sgRNA scaffold.


Usage and Biology

iGEM23_SCUT-China

In 2023, SCUT-China successfully applied the CRISPRi system containing this scarfold in a novel strain of Streptomyces sp. SCUT-3 and tested it effective. Details of the usage can be found in BBa_K4621080.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None