Device

Part:BBa_K3165051

Designed by: Mohit Das   Group: iGEM19_IISc-Bangalore   (2019-10-20)
Revision as of 12:13, 20 October 2019 by Registry (Talk | contribs)

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(N-Terminal T7 RNAP Domain + nMag) Generator (for Escherichia coli)

This part encodes for the N-terminal T7 RNA Polymerase domain attached to the nMag photo-sensitive unit via linker sequence under a ribosome binding site with a double terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1681
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 904
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1353


[edit]
Categories
Parameters
None