Part:BBa_K2985009
Pul-> Amylopectin 6-glucan hydrolase
Pul gene encodes a protein, pullulanase, which can specifically hydrolyze the alpha-1,6 glycoside bonds in amylopectin branching points by endonucleation, thus cutting down the whole branching structure to form amylose amylopectin debranching enzymes. We use these enzymes as a tool for our characterization of promoters.
SDS-page detection qualitative analysis
Methods and results
Take 1 mL fermented liquid, in the 12000r∙min-1, 1 min the centrifugal, the fermented supernatant as samples to identify the Protein SDS-page electrophoresis of pullulan enzyme expression, using SDS-PAGE of concentrated gum concentration was 5%, the concentration of separation gel was 9%, take samples of 30 mu, 4 x L SDS-page Protein Loading Buffer 10 mu L and blending boil after 5 min, 5000 r, 2 min min-1 centrifugal, samples from 15 mu L processed Protein to join the fibrin glue lane, 5 μl high molecular weight protein Marker was added.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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