Regulatory
miR663b-ta
Part:BBa_K3179000:Design
Designed by: Jiaxian Xu Group: iGEM19_SYSU-CHINA (2019-10-13)
miR663b-target
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We constructed miR663b-target with 2kt-E1A and miR885-5p-target so that E1A cannot be translated if a high level of miRNA663b existing in the cell.
Source
MiRNA 663b-target is constructed bearing four repeats of miRNA 663b, and each repeat added with ATG. Extra bases which can be devisable by three were added in order to separate ATGs.
References
1 Bartel D P. MicroRNAs: target recognition and regulatory functions[J]. cell, 2009, 136(2): 215-233.