Regulatory
miR663b-ta

Part:BBa_K3179000:Design

Designed by: Jiaxian Xu   Group: iGEM19_SYSU-CHINA   (2019-10-13)


miR663b-target


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part, four repeating sequences complementary to miRNA663b on an mRNA, is a 4X miRNA target designed to target miRNA663b. When miRNA663b reaches a certain concentration in the cell, it would combine with miR663b-target and suppress the translation of mRNA. According to our design, miR663b-target is designed to control the translation of mRNA containing response parts. We constructed miR663b-target with 2kt-E1A and miR885-5p-target so that E1A cannot be translated if a high level of miRNA663b existing in the cell.

Source

We get this sequence from NCBI, and the sequence was synthesized from a biological company.

References

1 Bartel D P. MicroRNAs: target recognition and regulatory functions[J]. cell, 2009, 136(2): 215-233.