Coding

Part:BBa_K2924035

Designed by: Andreas Nakielski   Group: iGEM19_Duesseldorf   (2019-10-11)
Revision as of 14:22, 19 October 2019 by AndreasN (Talk | contribs) (→‎Usage and Biology)


mVenus Fluorescent protein mVenus, codon optimized for Synechocystis sp. PCC 6803

Usage and Biology

mVenus is a yellow fluorescent protein derived from gfp and its derivative eYFP, which stem from Aequorea victoria. Its peak excitation wavelength is at 515 nm and it emits light of 527 nm peak wavelength1. The protein has a size of 26.9 kDa and is usually found as a monomer.

This part was characterized with the Pcpc560 Promoter in terms of fluorescence, protein product and used to test a CRIPRi-dCas9 gene knock-down in the composite part BBa_K2924036. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 630
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 28
    Illegal AgeI site found at 151
  • 1000
    COMPATIBLE WITH RFC[1000]


References

1: Kremers, Gert-Jan, et al. "Cyan and yellow super fluorescent proteins with improved brightness, protein folding, and FRET Förster radius." Biochemistry 45.21 (2006): 6570-6580.

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