Composite
INT5TLU1

Part:BBa_K3254026

Designed by: Yeqing Zong, DENG,Gabriel Cheuk Yan   Group: iGEM19_GENAS_China   (2019-10-18)
Revision as of 05:36, 18 October 2019 by Zongyeqing (Talk | contribs)


Translational Unit for Int5 integrase

This part can be placed downstreamed a prmoter. It is sure that when it placed under a Ptac promoter on a P15A plasmid and full induced, the recombination between Int5 attP and attB will happen as expected. The RiboJ(BBa_K2615996) part was added before the RBS-coding sequence, so that the modularity and reuseability would be improved.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 131
    Illegal PstI site found at 1525
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 131
    Illegal PstI site found at 1525
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 126
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 131
    Illegal PstI site found at 1525
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 131
    Illegal PstI site found at 1525
    Illegal AgeI site found at 118
    Illegal AgeI site found at 502
    Illegal AgeI site found at 877
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/recombination
Parameters
None