Part:BBa_K3254026

Translational Unit for Int5 integrase

This part can be placed downstreamed a prmoter. It is sure that when it placed under a Ptac promoter on a P15A plasmid and full induced, the recombination between Int5 attP and attB will happen as expected. The RiboJ(BBa_K2615996) part was added before the RBS-coding sequence, so that the modularity and reuseability would be improved.

Usage

The composition and principle of the experimental system

We placed this part downstreamed a Ptac promoter(BBa_K2572025) on a P15A plasmid, and it can cause the recombinant between its corresponding att sites (see BBa_K3254001) on a pSB4C5 plasmid when full induced. The information about orthogonality characterization (include experimental setup) can be found on the page of Int5 integrase (BBa_K3254006).

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 131
  Illegal PstI site found at 1525
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 131
  Illegal PstI site found at 1525
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 126
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 131
  Illegal PstI site found at 1525
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 131
  Illegal PstI site found at 1525
  Illegal AgeI site found at 118
  Illegal AgeI site found at 502
  Illegal AgeI site found at 877
  
• 1000
  COMPATIBLE WITH RFC[1000]