Coding
Part:BBa_K2926057
Designed by: Johanna Opgenoorth Group: iGEM19_Bielefeld-CeBiTec (2019-10-15)
Revision as of 22:54, 17 October 2019 by Jopgenoorth (Talk | contribs)
Fusion protein of mating factor alpha, mCherry and pVIII with his-tag
Mating factor alpha from S. cerevisiae was fused n-terminally to the red fluorescent protein mCherry. Additionally the M13 bacteriophages major coat protein pVIII was fused to the c-terminus of mCherry. To enable easy purificatio of the fusion protein a hexahistidine tag was fused to the c-terminus.
Sequence and Features<h1></span>
Sequence was validated by Sanger sequencing.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
| None |