Coding

Part:BBa_K2926057

Designed by: Johanna Opgenoorth   Group: iGEM19_Bielefeld-CeBiTec   (2019-10-15)


Fusion protein of mating factor alpha, mCherry and pVIII with his-tag

Mating factor alpha from S. cerevisiae was fused N-terminally to the red fluorescent protein mCherry. Additionally the M13 bacteriophages major coat protein pVIII was fused to the C-terminus of mCherry. To enable easy purification of the fusion protein a hexahistidine tag was fused to the C-terminus.


Sequence and Features

Sequence was validated by Sanger sequencing.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None