Coding

Part:BBa_K2926058

Designed by: Johanna Opgenoorth   Group: iGEM19_Bielefeld-CeBiTec   (2019-10-15)
Revision as of 21:48, 17 October 2019 by Jopgenoorth (Talk | contribs)


Fusion protein of Flo11 (yeast), mCherry and pVIII (M13 bacteriophage) with purification tag To visualize the interactions of the n-terminal lectin binding domain of the S. cerevisiae protein Flo11 we fused the coding sequence n-terminally to the coding sequence of the fluorescence reporter protein mCherry. mCherry was n-terminally fused to the M13 bacteriophage major coat protein pVIII. The whole protein was fused to a C-terminal hexahistidine tag to enable protein purification via metal ions.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None