Part:BBa_K2926058

Fusion protein of Flo11 (yeast), mCherry and pVIII (M13 bacteriophage) with purification tag To visualize the interactions of the n-terminal lectin binding domain of the S. cerevisiae protein Flo11 we fused the coding sequence N-terminally to the coding sequence of the fluorescence reporter protein mCherry. mCherry was N-terminally fused to the M13 bacteriophage major coat protein pVIII. The whole protein was fused to a C-terminal hexahistidine tag to enable protein purification via metal ions.

Sequence and Features

Sequence was validated by Sanger sequencing.

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]