Composite

Part:BBa_K2934006:Design

Designed by: Nir Litver   Group: iGEM19_Technion-Israel   (2019-10-10)
Revision as of 16:34, 17 October 2019 by NirLitver (Talk | contribs) (→‎Source)


AmyE - Invertase-Histag for Bacillus subtilis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 989
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1294
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 595
    Illegal BsaI.rc site found at 937
    Illegal SapI.rc site found at 1197


Design Notes

The combination was planned and made in our lab using the pBE-S commercial plasmid made by TaKaRa (B. subtilis Secretory Protein Expression System) [1].


Source

Invertase - BBa_K2934001

AmyE signal peptide - BBa_K2273023

linker - BBa_K2934005

References

[1] https://www.takarabio.com/products/protein-research/expression-vectors-and-systems/b-subtilis-expression-system