DNA

Part:BBa_K2961001:Design

Designed by: Muhammad Nahin Khan   Group: iGEM19_CMUQ   (2019-10-13)
Revision as of 01:34, 17 October 2019 by Mnahinkhan (Talk | contribs) (→‎Design Notes)


CFTR Wildtype gene segment


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

he fixed loop part was adapted from the NEB website, as per their instructions (https://international.neb.com/faqs/2018/05/03/how-do-i-design-a-guide-rna-for-use-with-engen-lba-cas12a). The segment that is specific to the CFTR gene wild type variant was generated through CHOPCHOP by Harvard, and verified through BLAST and other genome browsing softwares.

References