Coding
Aad9

Part:BBa_K143031:Design

Designed by: Chris Hirst   Group: iGEM08_Imperial_College   (2008-09-16)
Revision as of 15:43, 9 October 2008 by Chris (Talk | contribs)

Aad9 Spectinomycin Resistance Gene



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 488

Design Notes

The sequence of B. subtilis integration vector pDR111 was searched for the spectinomycin resistance gene and the Biobrick standard applied to the gene sequence upon PCR cloning

Source

Aad9 was PCR cloned from the B. subtilis integration vector pDR111 using Pfu DNA polymerase