Part:BBa_K143031:Design

Aad9 Spectinomycin Resistance Gene

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 488
  

Design Notes

The sequence of B. subtilis integration vector pDR111 was searched for the spectinomycin resistance gene and the Biobrick standard applied to the gene sequence upon PCR cloning

Template

B. subtilis integration plasmid pDR111#1

Forward Primer

5'- GCT CTA GAT GAG GAG GAT ATA TTT GAA TAC - 3'

Reverse Primer

5'- GGA CTA GTA TTA TTA TAA TTT TTT TAA TCT GTT ATT TAA ATA G -3'

Source

Aad9 was PCR cloned from the B. subtilis integration vector pDR111 using Pfu DNA polymerase

References

<biblio>

1. 1 pmid=12562810

</biblio>