Coding

Part:BBa_K2984038

Designed by: Darius Rauch   Group: iGEM19_Humboldt_Berlin   (2019-10-16)
Revision as of 14:26, 16 October 2019 by Registry (Talk | contribs)

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L1c-Psad-CBA-Rbcs2

L1c plasmid containing the gene for PETase enzyme fused to the phosphite oxidoreductase (ptxD) to create dependency on PETase expression when C.reinhardtii dwells in tris-acetate-phosphite media.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1106
    Illegal PstI site found at 1453
    Illegal PstI site found at 1826
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1106
    Illegal PstI site found at 1453
    Illegal PstI site found at 1826
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4
    Illegal BamHI site found at 1085
    Illegal XhoI site found at 1271
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1106
    Illegal PstI site found at 1453
    Illegal PstI site found at 1826
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1106
    Illegal PstI site found at 1453
    Illegal PstI site found at 1826
    Illegal NgoMIV site found at 1208
    Illegal NgoMIV site found at 2095
    Illegal AgeI site found at 2036
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None