Coding

Part:BBa_K2984038

Designed by: Darius Rauch   Group: iGEM19_Humboldt_Berlin   (2019-10-16)


L1c-Psad-CBA-Rbcs2


This vector is a part of the Chlamy-HUB-Collection. This L1 part contains our carbonic anhydrase sequence, a PsaD Promotor and our Rbcs Terminator. However we didn´t do any transformations in chlamydomonas reinhardtii or characterizations with this part because we had to prioritize other constructs.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1106
    Illegal PstI site found at 1453
    Illegal PstI site found at 1826
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1106
    Illegal PstI site found at 1453
    Illegal PstI site found at 1826
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4
    Illegal BamHI site found at 1085
    Illegal XhoI site found at 1271
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1106
    Illegal PstI site found at 1453
    Illegal PstI site found at 1826
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1106
    Illegal PstI site found at 1453
    Illegal PstI site found at 1826
    Illegal NgoMIV site found at 1208
    Illegal NgoMIV site found at 2095
    Illegal AgeI site found at 2036
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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