Regulatory
PtacOpt

Part:BBa_K3254014

Designed by: Yeqing Zong   Group: iGEM19_GENAS_China   (2019-10-16)
Revision as of 09:03, 16 October 2019 by Registry (Talk | contribs)

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Optimized Ptac promoter

This promoter was optimized from Ptac promoter(BBa_K864400) through using a symmetrical lacO site. After combined with the lacI constitutive expression cassette(BBa_K3254022), it can be regulated by the IPTG inducer. A mutant library with different dynamic range had been built(see BBa_K3254015,BBa_K3254016 and BBa_K3254017).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
//direction/forward
//promoter
//regulation/positive
//rnap/prokaryote/ecoli/sigma70
Parameters
None