Composite

Part:BBa_K3096053

Designed by: Jakob Keck, Patrick Mueller, Lina Widerspick, Famke Baeuerle   Group: iGEM19_Tuebingen   (2019-10-15)
Revision as of 15:20, 15 October 2019 by Famosa (Talk | contribs)

CRISPR Array targeting the Plasmid DNA of the Exendin-4 expression system controlled by Lsr

The CRISPR array sequence needed to guide the Cas3 nuclease to its cut sites on the plasmid with the glucose dependent Exendin-4 expression system under the control of the LsrR promoter. When LsrR is present the array is not transcribed and the CRISPR/Cas3 System lacks its gRNA and is inactive. Combined with the fatty acid responsive repression system (BBa_K3096046) the expression of the array can be controlled.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[edit]
Categories
Parameters
None