Composite
Part:BBa_K3096053
Designed by: Jakob Keck, Patrick Mueller, Lina Widerspick, Famke Baeuerle Group: iGEM19_Tuebingen (2019-10-15)
CRISPR Array targeting the Plasmid DNA of the Exendin-4 expression system controlled by Lsr
The CRISPR array sequence needed to guide the Cas3 nuclease to its cut sites on the plasmid with the glucose dependent Exendin-4 expression system under the control of the LsrR promoter. When LsrR is present the array is not transcribed and the CRISPR/Cas3 System lacks its gRNA and is inactive. Combined with the fatty acid responsive repression system (BBa_K3096046) the expression of the array can be controlled.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
- Xia, Peng-Fei, et al. "Synthetic genetic circuits for programmable biological functionalities." Biotechnology advances (2019).
- Terns, Michael P., and Rebecca M. Terns. "CRISPR-based adaptive immune systems." Current opinion in microbiology 14.3 (2011): 321-327.
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Categories
Parameters
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