Regulatory

Part:BBa_K3096011

Designed by: Famke Baeuerle, Patrick Mueller, Lina Widerspick, Jakob Keck   Group: iGEM19_Tuebingen   (2019-10-14)
Revision as of 14:32, 15 October 2019 by Lina widerspick (Talk | contribs)


CAP promoter without LacI binding site

CAP promoter regulates expression in dependency of glucose. When glucose levels are low, the promoter is active.


Usage and Biology

This part originates from the Lactose operon. The operator - binding site of LacI - was deleted to yield a lactose independent regulatory system. It is known that the Lactose operon also responds to catabolite repression, which is now the only regulatory element within the promoter, making it solely dependent on glucose. If the glucose concentration is LOW, the bacterial Adenylate Cyclase is active and synthesises cAMP (cyclic AMP), which interacts with CAP (catabolite activating protein). The CAP-cAMP complex then binds the CAP binding site activating transcription. If the glucose concentration increases, the system will be repressed. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None