Part:BBa_K3096011

CAP promoter without LacI binding site

CAP promoter regulates expression in dependency of glucose. When glucose levels are low, the promoter is active.

Usage and Biology

This part originates from the Lactose operon. The operator - binding site of LacI - was deleted to yield a lactose independent regulatory system. It is known that the Lactose operon also responds to catabolite repression, which is now the only regulatory element within the promoter, making it solely dependent on glucose.

If the glucose concentration is LOW, the bacterial Adenylate Cyclase is active and synthesises cAMP (cyclic AMP), which interacts with CAP (catabolite activating protein). The CAP-cAMP complex then binds the CAP binding site activating transcription. If the glucose concentration increases, the system will be repressed.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]