Composite

Part:BBa_K3112043

Designed by: Cheng Chen   Group: iGEM19_Tianjin   (2019-10-14)
Revision as of 17:21, 14 October 2019 by Registry (Talk | contribs)

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mRFP+HIS3 Chromosome Horizontal Transfer Elements

This part was designed to transform mRFP into Saccharomyces cerevisiae genome.

The mRFP in the part was codon optimized for being suitable for Saccharomyces cerevisiae.

The final significance is to characterize the horizontal transfer of Saccharomyces cerevisiae chromosomes in the fusion system and to confirm its expression in the fusion system.

Before fusion, we also characterize its function to prove its validity.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2286
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2177
    Illegal BglII site found at 2237
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1576
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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