Part:BBa_K3112043

mRFP+HIS3 Chromosome Horizontal Transfer Elements

This part was designed to transform mRFP into Saccharomyces cerevisiae genome.

The mRFP in the part was codon optimized for being suitable for Saccharomyces cerevisiae.

The final significance is to characterize the horizontal transfer of Saccharomyces cerevisiae chromosomes in the fusion system and to confirm its expression in the fusion system.

Before fusion, we also characterize its function to prove its validity.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 2286
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 2177
  Illegal BglII site found at 2237
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1576
  
• 1000
  COMPATIBLE WITH RFC[1000]

We transformed mRFP into the genome of Saccharomyces cerevisiae and expressed it, and photographed it with a fluorescence microscope before and after fusion to confirm whether this chromosome has metastasized.

Figure 1. Photos before and after fluorescence excitation under fluorescence microscope

If red fluorescence is detected from the fusion system after red light excitation, then the entire chromosome can be proved to have metastasized without being digested.