Composite
Part:BBa_K3110001
Designed by: Mrugank Dake Group: iGEM19_IISER_Tirupati (2019-10-13)
lldR+lldD
This is a combination of lldR (the regulatory protein of lldPRD) which has a dual role of activator and repressor; and lldD (L-Lactate Dehydrogenase) which cleave L-Lactate to Pyruvate. Our intention of using this part is to have a control over the detection threshold of L-Lactate by the lldPRD regulatory region.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1929
Illegal AgeI site found at 567 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1366
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Categories
Parameters
None |