Composite

Part:BBa_K3110001

Designed by: Mrugank Dake   Group: iGEM19_IISER_Tirupati   (2019-10-13)


lldR+lldD

This is a combination of lldR (the regulatory protein of lldPRD) which has a dual role of activator and repressor; and lldD (L-Lactate Dehydrogenase) which cleaves L-Lactate to Pyruvate. Our intention of using this part is to have control over the detection threshold of L-Lactate by the lldPRD regulatory region.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1929
    Illegal AgeI site found at 567
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1366


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Categories
Parameters
None