RNA

Part:BBa_K2923003

Designed by: claire husser   Group: iGEM19_Strasbourg   (2019-09-12)
Revision as of 09:55, 13 October 2019 by Katya (Talk | contribs)


Guanine actif aptazyme

An aptazyme is an aptamer-ribozyme combination with intrinsic RNA cleavage activity. this aptazyme is specific for the guanine (ligand). It is composed of 3 different parts: a twister ribozyme module, a communication module, and an aptamer module. This aptazyme is an ON-switches aptazyme. In the presence of ligand, the guanine aptazyme provides self-cleave. In-vivo ligand of guanine aptazyme is guanosine.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//classic/rna/uncategorized
Parameters
ligandsin-vitro assay : Guanine; in-vivo assay Guanosine
switch_pointThe maximum effect obtained at approximately 500 μM of guanosine