Plasmid

Part:BBa_I732954

Designed by: Zhan Jian   Group: iGEM07_USTC   (2007-10-26)
Revision as of 01:25, 14 September 2008 by Rshetty (Talk | contribs)

Ptemplate1 + RBS.ARL4A0604

Genotype Character Name
Top10/pT-TERM So not express any repressors CR00
Top10/pT-ARL4A0604 Constitutively express ARL4A0604 CR01
Top10/pT-ARL2A0203 Constitutively express ARL2A0203 CR10
Top10/pTet-ARL4A0604-ARL2A203 Constitutively express ARL4A0604 and ARL2A0203 CR11

USTC plasmid 954.jpg


  • RS 01:25, 14 September 2008 (UTC): Note that this plasmid has been split into a plasmid backbone and part. The plasmid backbone is BBa_I732952 and the part is I732815. The physical DNA associated with this part has sequence BBa_I732952-I732815. This part needs to be deleted and the physical DNA remapped to the correct part number.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 1105
    Illegal EcoRI site found at 3240
    Illegal XbaI site found at 3405
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3240
    Illegal SpeI site found at 1106
    Illegal PstI site found at 1120
    Illegal NotI site found at 1113
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3240
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 1106
    Illegal EcoRI site found at 3240
    Illegal XbaI site found at 3405
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3240
    Illegal XbaI site found at 3405
    Illegal SpeI site found at 1106
    Illegal PstI site found at 1120
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2279
    Illegal BsaI.rc site found at 3264


[edit]
Categories
Parameters
chassis
copies
mcsBioBrick friendly
origin
resistanceA