Coding

Part:BBa_K2972001:Design

Designed by: Huanzhou Yang   Group: iGEM19_BIT-China   (2019-10-12)
Revision as of 03:01, 13 October 2019 by Registry (Talk | contribs)

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Sigma F


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 101


Design Notes

The two ends of this sequence are highly similar to sigmaB. It may lead to the failure of PCR.


Source

It come from Bacillus subtilis genomic sequence.

References