Coding

Part:BBa_K2972001:Design

Designed by: Huanzhou Yang   Group: iGEM19_BIT-China   (2019-10-12)


Sigma F


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 101


Design Notes

Make sure it is orthogonal to the host cell before use.

Source

Sigma F is derived from Strains B. subtilis subsp. subtilis wild-type (LMG 7135), and it’s a regulatory protein that is responsible for directing gene expression in the forespore compartment of developing cells of the spore-forming soil bacterium Bacillus subtilis.

References