Composite

Part:BBa_K3105683:Design

Designed by: Jonas Gockel   Group: iGEM19_Uppsala_Universitet   (2019-10-12)
Revision as of 20:31, 12 October 2019 by Registry (Talk | contribs)

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Expression construct Glox


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1825
    Illegal BamHI site found at 2529
    Illegal XhoI site found at 1183
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 3014
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

For this design, overhangs complementary to the pPICZαB vector (easy select pichia expression kit from invitrogen) were added by PCR. This was then assembled into the expression vector generating the circuit in this part


Source

see basic parts

References