Composite

Part:BBa_K3105683:Design

Designed by: Jonas Gockel   Group: iGEM19_Uppsala_Universitet   (2019-10-12)


Expression construct Glox


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1825
    Illegal BamHI site found at 2529
    Illegal XhoI site found at 1183
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 3014
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

For this design, overhangs complementary to the pPICZαB vector (Invitrogen easy select Pichia expression kit) were added to BBa_K500003 by PCR. This was then assembled into the expression vector generating the circuit in this part

Source

see basic parts

References