Coding

Part:BBa_K2951000

Designed by: YU-RU LIN   Group: iGEM19_CSMU_Taiwan   (2019-07-18)
Revision as of 18:13, 12 October 2019 by Yuruim (Talk | contribs)

Influenza A virus nucleocapsid protein

Short Description

This part contains the original sequence of Influenza A nucleocapsid protein(NP) obtained from NCBI.

Characterizaiton

This part was characterized by CSMU_Taiwan 2019.Because of its stringent sequence specificity, tobacco etch virus (TEV) protease emerges as a useful reagent with wide application in the cleavage of recombinant fusion proteins. Thus, we have chosen TEV protease(sequence from BBa_K1319004) to remove the fusion protein in our another part BBa_K2951008 and be characterized.

Small scale production

Cultivations and Induction of protein expression

Large scale production

Cultivations and Induction of protein expression

Protein solubility analysis

Purification and Dialysis

Enzyme activity assay

References

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 477
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 238


[edit]
Categories
Parameters
None