Part:pSB6A1:Design

pBR322 converted to BioBrick vector (formally BBa_I739201)

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 4001
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  Illegal NotI site found at 9
  Illegal NotI site found at 4007
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 4001
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found at 4001
  Illegal suffix found at 2
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found at 4001
  Plasmid lacks a suffix.
  Illegal XbaI site found at 4016
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  Illegal NgoMIV site found at 43
  Illegal NgoMIV site found at 411
  Illegal NgoMIV site found at 571
  Illegal NgoMIV site found at 925
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI.rc site found at 3075
  Illegal SapI site found at 1992
  

Design Notes

• RS 23:11, 3 September 2008 (UTC): The default insert for this vector is Part:BBa_I739400. The physical location for this vector needs to be remapped to Part:BBa_I739400. pSB6A1 is the vector that contains Part:BBa_I739400.

Source

Bioprocess Laboratory at ETH Zurich

References

<biblio>

1. Bolivar-Gene-1977 pmid=344137

</biblio>