Coding

Part:BBa_K2963018

Designed by: Experiment group members of JNU-China   Group: iGEM19_JNU-China   (2019-09-12)
Revision as of 07:21, 12 October 2019 by GHX (Talk | contribs)


pgsB - encoding a subunit of poly-γ-glutamic acid synthetase

This part contains the pgsB gene for poly-gamma-glutamic acid synthetase. This enzyme is involved in poly-gamma-glutamic acid synthesis,which converts the glutamate to poly-gamma-glutamic acid. PgsB is mainly responsible for catalysing poly-gamma-glutamic acid synthesis. In our subject, we get the pgsB sequenece by error-prone PCR of Bacillus genome, trying to improve the activity of pgsB protein.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1032
    Illegal PstI site found at 1013
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1013
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1032
    Illegal PstI site found at 1013
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1032
    Illegal PstI site found at 1013
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None