Coding
Part:BBa_K2963018
Designed by: Experiment group members of JNU-China Group: iGEM19_JNU-China (2019-09-12)
pgsB - encoding a subunit of poly-γ-glutamic acid synthetase
This part contains the pgsB gene which encodes a subunit for poly-γ-glutamic acid synthetase. This enzyme is involved in poly-γ-glutamic acid synthesis, which converts the glutamate to poly-γ-glutamic acid. PgsB is mainly responsible for catalysing poly-γ-glutamic acid synthesis. In our subject, we get the pgsB sequenece from Bacillus sp. genome.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 1032
Illegal PstI site found at 1013 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 1013
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 1032
Illegal PstI site found at 1013 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 1032
Illegal PstI site found at 1013 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |