Composite

Part:BBa_K3105677:Design

Designed by: Jonas Gockel   Group: iGEM19_Uppsala_Universitet   (2019-10-11)
Revision as of 22:01, 11 October 2019 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


Expression construct HRP-2A-eGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2086
    Illegal XhoI site found at 1183
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2167
    Illegal AgeI site found at 3176
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

AOX1 promoter inducible with methanol was used to achieve high expression as well as tight control of the expression system. α-factor secretion signal was used due to


Source

See basic parts

References