Part:BBa_K3105677:Design

Expression construct HRP-2A-eGFP

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 2086
  Illegal XhoI site found at 1183
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 2167
  Illegal AgeI site found at 3176
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

AOX1 promoter inducible with methanol was used to achieve high expression as well as tight control of the target protein.
α-factor secretion signal was used to enable secretion of target enzyme for easy purification from the supernatant.

Source

See basic parts