Composite

Part:BBa_K2934004

Designed by: Nir Litver   Group: iGEM19_Technion-Israel   (2019-10-10)
Revision as of 14:38, 10 October 2019 by NirLitver (Talk | contribs)


AmyE - Glucose Oxidase-Histag for Bacillus subtilis

This part contains the Glucose Oxidase (GOx) gene (BBa_K2934000), connected to the secretory signal peptide AmyE (from the alpha-amylase gene) (BBa_K2273023), using a short nine amino-acids linker (BBa_K2934005).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 156
    Illegal BsaI site found at 487
    Illegal BsaI.rc site found at 433
    Illegal BsaI.rc site found at 1258


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Categories
Parameters
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