Part:BBa_K3102014

LldP (L-lactate permease)

E.coli, lldP is an inner membrane permease that functions in the uptake of L-lactate, D-lactate and glycolate [Dong et al., 1993; Nunez et al., 2002]. The lldP gene is located in a lactate-inducible operon with the lldD and lldR genes encoding a lactate dehydrogenase and a regulatory protein, respectively. By overexpressing a plasmid carrying this enzyme, the bacteria is able to uptake the double of these three substrates, L-lactate, D-lactate and glycolate.

Dong JM et al., Three overlapping lct genes involved in L-lactate utilization by Escherichia coli., Journal of Bacteriology, 1993 Oct;175(20):6671-8.

Nunez MF et al., Transport of L-Lactate, D-Lactate, and glycolate by the LldP and GlcA membrane carriers of Escherichia coli, Biochem Biophys Res Commun. 2002 Jan 18;290(2):824-9.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 275
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 411
  Illegal AgeI site found at 874
  Illegal AgeI site found at 1312
  Illegal AgeI site found at 1471
  Illegal AgeI site found at 1483
  
• 1000
  COMPATIBLE WITH RFC[1000]