Coding

Part:BBa_K3102014

Designed by: Sarah DUQUESNE   Group: iGEM19_Ionis_Paris   (2019-10-03)


LldP (L-lactate permease)

E.coli, lldP is an inner membrane permease involved in the uptake of L-lactate, D-lactate and glycolate [Dong et al., 1993; Nunez et al., 2002]. The lldP gene is located in a lactate-inducible operon with the lldD and lldR genes encoding a lactate dehydrogenase and a regulatory protein, respectively. By overexpressing a plasmid carrying this enzyme, the bacteria is able to uptake twice the amount of these three substrates, L-lactate, D-lactate and glycolate.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 275
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 411
    Illegal AgeI site found at 874
    Illegal AgeI site found at 1312
    Illegal AgeI site found at 1471
    Illegal AgeI site found at 1483
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None