Composite

Part:BBa_K3044009

Designed by: Catharina Bang Jensen   Group: iGEM19_SDU-Denmark   (2019-10-06)
Revision as of 18:54, 6 October 2019 by Anne Sofie (Talk | contribs)


dCas9 with IPTG inducible promotor

This dCas9 is a codon optimized version of BBa_K1150000 for expression in E. coli. Together with a single guide RNA (sgRNA), the protein can be used for silencing of specific target genes. dCas9 is the inactive form of the endonuclease Cas9. In contrast to Cas9, dCas9 does not cleave the DNA. Instead, it sterically blocks the elongation of transcription as a result of the sgRNA-DNA interaction. This will only repress the expression of the target gene thus avoiding the destruction of the gene (1).

The dCas9 is asembled with the IPTG inducible promoter BBa_R0011 and an RBS BBa_B0034 making the expression of the protein controlable.

[1]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 332
    Illegal BglII site found at 1406
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None