Coding

Part:BBa_K3102008

Designed by: Chloe DOIZELET   Group: iGEM19_Ionis_Paris   (2019-10-03)
Revision as of 21:02, 3 October 2019 by Registry (Talk | contribs)

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ZWF (Glucose-6-phosphate 1-dehydrogenase)

ZWF (Glucose-6-phosphate 1-dehydrogenase) catalyzes the oxidation of glucose 6-phosphate to 6-phosphogluconolactone in the pentose phosphate pathway. (Due to the knock-out of PGI, there is a metabolic flux shift toward the pentose phosphate shunt using this enzyme to allow the bacteria to survive.) This part is useful to increase flavin production.

"Effect of zwf gene knockout on the metabolism of Escherichia coli grown on glucose or acetate." Zhao J., Baba T., Mori H., Shimizu K. Metab. Eng. 6:164-174(2004)


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 775
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 775
    Illegal NotI site found at 656
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1332
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 775
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 775
    Illegal NgoMIV site found at 48
    Illegal NgoMIV site found at 765
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None