Coding

Part:BBa_K3102008

Designed by: Chloe DOIZELET   Group: iGEM19_Ionis_Paris   (2019-10-03)


ZWF (Glucose-6-phosphate 1-dehydrogenase)

ZWF (Glucose-6-phosphate 1-dehydrogenase) catalyzes the oxidation of glucose 6-phosphate to 6-phosphogluconolactone in the pentose phosphate pathway. (Due to the knock-out of PGI, there is a metabolic flux shift toward the pentose phosphate shunt using this enzyme to allow the bacteria to survive.) This part is used to increase flavin production.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 775
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 775
    Illegal NotI site found at 656
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1332
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 775
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 775
    Illegal NgoMIV site found at 48
    Illegal NgoMIV site found at 765
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None