Coding
ARS B2-B2

Part:BBa_K2984000:Design

Designed by: Sophia Schiebler   Group: iGEM19_Humboldt_Berlin   (2019-10-02)
Revision as of 19:59, 2 October 2019 by Ropionju (Talk | contribs) (added Rasala reference)


3'UTR, Secretion signal of Arylsulfatase ARS B2-B2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This was translated to fit the codon usage of C. reinhardtii.


Source

It is an endogenous secretion signal of C. reinhardtii's nuclear genome.

References

Rasala, B. A., Lee, P. A., Shen, Z., Briggs, S. P., Mendez, M., & Mayfield, S. P. (2012). Robust Expression and Secretion of Xylanase1 in Chlamydomonas reinhardtii by Fusion to a Selection Gene and Processing with the FMDV 2A Peptide. PLOS ONE, 7(8), e43349. Retrieved from https://doi.org/10.1371/journal.pone.0043349